DIA can't demultiplex | wendy white | 2020-06-18 11:35:55 | |||||||||
I made a DIA overlap method on the instrument- but when I use MS Convert GUI, it fails and reports this error: ------------------------------ Starting... Opening file "C:\Users\WHITEW01\Desktop\Histones\2020_0617_histones.raw" for read... Calculating SHA1 checksum... Processing... Writing "C:\Users\WHITEW01\Desktop\Histones\2020_0617_histones.mzML"... Failed - System.Exception: SpectrumToIndices() Number of demultiplexing windows changed. Minimum window size or window boundary tolerance may be set too low. at pwiz.CLI.msdata.MSDataFile.write(MSData msd, String filename, WriteConfig config, IterationListenerRegistry iterationListenerRegistry) at MSConvertGUI.MainLogic.processFile(String filename, Config config, ReaderList readers, Map`2 usedOutputFilenames) at MSConvertGUI.MainLogic.Go(Config config, Map`2 usedOutputFilenames). |
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