Stack Overflow Error Message - 7/9/2024

The mechanism used to create methods from a template has failed on occasion with a Stack Overflow message. This can happen with method export from:

• Skyline using File / Export / Method
• GPF Creator when creating sets of DIA methods
• PRM Conductor when exporting an assay to a targeted method

We think that the Thermo method export library loads certain assemblies, and that it is possible that another process loads them first and renders them unusable by our programs. Running our processes as an administrator seems to fix the problem across multiple launches of Skyline.

Solution 1: Running Skyline as an Administrator

Select Tools / Thermo / GPF Creator to launch this program. In Skyline, the immediate window opens that displays the path to GPF Creator.

Copy paste this path into a windows explorer path, removing the parenthesis " before the C:\Users. Go up one level past the Tools folder, and sort the files by Type, and the Skyline-Daily.exe will be visible. Close the current instance of Skyline and right click the executable and run as an Administrator.

Solution 2: Running GPF Creator as an Administrator

We think that Solution 1 will work, but on a previous instance, before I had realized that Skyline-Daily.exe was in the folder one up from Tools, I fixed the problem by running GPF Creator as an administrator and exporting a method. This allowed methods to be exported from Skyline, GPF Creator, or PRM Conductor across multiple program launches and computer restarts.

• Like for Solution 1, open a windows explorer folder but this time in the folder where GPF Creator is.

• Right-click the Thermo.TNG.PRMBuilder.SurveyCreator and run as an Administrator.

• The GPF Creator application will launch.

• Select a method that has a DIA experiment in it. We have attached a dia method, but you can also easy build one, but opening method editor, selecting the Hybrid view button, and double-clicking the DIA Scan in the MSn menu on the left.

• This will add the DIA experiment to the time line. Save the file, and then in GPF Creator, double click the Method Template file box and select this file, and press the Create Method Files button. This should create the new template methods as normal, and free up the Thermo method export libraries for future calls.