Table 1. Study Results. Shown is the ratio of tryptic (L) to isotope-labeled peptide (H) for all participants included in Study 1 (AD N = 7 and controls N = 10), Study 2 (AD N = 12 and controls N = 14), Study 3 (AD N = 6, PD N = 10, prodromal AD N = 10 and stable MCI N = 15) and Study 4 (AD N = 36, PD N = 11 and controls N = 44).

Table 2. Proteins and peptides targeted using a combination of SPE and PRM-MS. Shown are the proteins and peptides targeted and information on the stable isotope labeled peptides used, methodological information and methodological performance.

Table 3. Calibration Curve Peptide Concentrations. Shown is the concentration of stable isotope-labeled peptides and proteins in a serially diluted mixture. After serial dilution an equal concentration of bovine serum albumin was added to each calibration point. No bovine serum albumin was added to calibration point 4 which therefore was excluded.

Table 4. Calibration Curve Data. The data show the area ratio of stable isotope-labeled peptide to tryptic peptide (H/L) from reverse calibration curves. In three experiments, duplicate curves were prepared by spiking dilution mixtures into a QC CSF sample. Six calibration points (1-3 and 5-7) and a blank (0), only containing bovine serum albumin protein, were analyzed.

Table 5. Precision Data. Eight QC CSF replicate samples were prepared and analyzed on five separate occasions. The table show the area ratio of tryptic to isotope-labeled heavy peptide (L/H) for each replicate and experiment.