Figure 4A.
In vitro kinase assay coupled to phosphorylation-dependent mobility shift analysis. Reactions were performed using recombinant hFLNc d18-21 WT in the presence (+) or absence (-) of ATP, Akt and/or PKCĪ± as indicated. Immunoblot analysis performed with an antibody directed against the EEF-tag that was fused to the carboxy-terminus of hFLNc d18-21 WT revealed multiple shifted bands, indicating kinase-dependent phosphorylation events. h, human; WT, wild-type