| Peptide Note/Annotations | | | | | Precursor Note/Annotations | | | | | | | |
|---|
 FKDLGEEHFK | Removed: false | 1 | 1248.6139 | |  FKDLGEEHFK | Removed: false | light | 2+ | 625.3142 | 1248.6139 | 11 | 24.5747 | |
FKDLGEEHFK | Removed: false | 1 | 1248.6139 | | FKDLGEEHFK | Note: Not sure why this precursor was the one remove, as it is slightly more intense.
Removed: true | light | 3+ | 417.2119 | 1248.6139 | 11 | 21.6713 | |
DLGEEHFK | Note: Fragment ion signal is higher than precursor ion signal, making this a questionable match.
Peptide Quality: Potentially
Removed: true | 0 | 973.4505 | | DLGEEHFK | Removed: false | light | 2+ | 487.7325 | 973.4505 | 8 | 19.8969 | |
LVNELTEFAK | Removed: false | 0 | 1162.6234 | | LVNELTEFAK | Removed: false | light | 2+ | 582.3190 | 1162.6234 | 11 | 23.1128 | |
TCVADESHAGCEK | Removed: false | 0 | 1462.5817 | | TCVADESHAGCEK | Removed: false | light | 2+ | 732.2981 | 1462.5817 | 11 | 28.2121 | |
TCVADESHAGCEK | Removed: false | 0 | 1462.5817 | | TCVADESHAGCEK | Note: Not sure why this precursor was removed.
Removed: true | light | 3+ | 488.5345 | 1462.5817 | 11 | 24.8095 | |
SLHTLFGDELCK | Removed: false | 0 | 1418.6864 | | SLHTLFGDELCK | Removed: false | light | 2+ | 710.3505 | 1418.6864 | 11 | 27.4659 | |
SLHTLFGDELCK | Removed: false | 0 | 1418.6864 | | SLHTLFGDELCK | Removed: false | light | 3+ | 473.9027 | 1418.6864 | 11 | 24.1657 | |
ETYGDMADCCEK | Removed: false | 0 | 1477.5160 | | ETYGDMADCCEK | Removed: false | light | 2+ | 739.7653 | 1477.5160 | 11 | 28.4660 | |
NECFLSHK | Note: Interference and high precursor mass error make this one questionable, but only in 100nM
Peptide Quality: Potentially
Removed: true | 0 | 1033.4651 | | NECFLSHK | Removed: false | light | 2+ | 517.7398 | 1033.4651 | 8 | 20.9172 | |
NECFLSHKDDSPDLPK | Removed: false | 1 | 1900.8625 | | NECFLSHKDDSPDLPK | Removed: false | light | 3+ | 634.6281 | 1900.8625 | 11 | 31.2376 | |
DDSPDLPK | Note: Product signal is much better at 40, 41 and 43 minutes, but no precursor signal. At 26 minutes there is precursor signal, but with 15 ppm mass error and less well defined product signal. This is at least likely a modified peptide and difficult to measure.
Peptide Quality: Potentially
Removed: true | 0 | 885.4080 | | DDSPDLPK | Removed: false | light | 2+ | 443.7113 | 885.4080 | 8 | 18.4002 | |
LKPDPNTLCDEFK | Removed: false | 0 | 1575.7603 | | LKPDPNTLCDEFK | Removed: false | light | 2+ | 788.8874 | 1575.7603 | 11 | 30.1362 | |
LKPDPNTLCDEFK | Removed: false | 0 | 1575.7603 | | LKPDPNTLCDEFK | Removed: false | light | 3+ | 526.2607 | 1575.7603 | 11 | 26.4695 | |
LKPDPNTLCDEFKADEK | Removed: false | 1 | 2018.9619 | | LKPDPNTLCDEFKADEK | Removed: false | light | 3+ | 673.9946 | 2018.9619 | 11 | 32.9698 | |
RHPYFYAPELLYYANK | Note: No believable precursor signal and low dotp value make this detection unlikely.
Peptide Quality: Bad
Removed: true | 1 | 2044.0207 | | RHPYFYAPELLYYANK | Removed: false | light | 3+ | 682.3475 | 2044.0207 | 11 | 33.3373 | |
HPYFYAPELLYYANK | Note: The most believable peak with idotp=0.99 and dotp=0.9 has low and poorly co-eluting signal on the product ions.
Peptide Quality: Potentially
Removed: true | 0 | 1887.9196 | | HPYFYAPELLYYANK | Removed: false | light | 3+ | 630.3138 | 1887.9196 | 11 | 31.0478 | |
AEFVEVTK | Removed: false | 0 | 921.4807 | | AEFVEVTK | Removed: false | light | 2+ | 461.7477 | 921.4807 | 9 | 19.0134 | |
ECCHGDLLECADDR | Removed: false | 0 | 1748.6553 | | ECCHGDLLECADDR | Removed: false | light | 3+ | 583.8924 | 1748.6553 | 11 | 29.0053 | |
ECCHGDLLECADDRADLAK | Removed: false | 1 | 2246.9355 | | ECCHGDLLECADDRADLAK | Removed: false | light | 3+ | 749.9858 | 2246.9355 | 11 | 36.3134 | |
YICDNQDTISSK | Removed: false | 0 | 1442.6348 | | YICDNQDTISSK | Removed: false | light | 2+ | 722.3247 | 1442.6348 | 11 | 27.8730 | |
LKECCDKPLLEK | Removed: false | 1 | 1531.7738 | | LKECCDKPLLEK | Removed: false | light | 2+ | 766.8942 | 1531.7738 | 11 | 29.3884 | |
LKECCDKPLLEK | Removed: false | 1 | 1531.7738 | | LKECCDKPLLEK | Removed: false | light | 3+ | 511.5986 | 1531.7738 | 11 | 25.8243 | |
ECCDKPLLEK | Removed: false | 0 | 1290.5948 | | ECCDKPLLEK | Removed: false | light | 2+ | 646.3047 | 1290.5948 | 9 | 25.2884 | |
ECCDKPLLEK | Removed: false | 0 | 1290.5948 | | ECCDKPLLEK | Note: Not sure why this precursor was removed.
Removed: false | light | 3+ | 431.2055 | 1290.5948 | 8 | 22.2870 | |
SHCIAEVEK | Removed: false | 0 | 1071.5019 | | SHCIAEVEK | Removed: false | light | 2+ | 536.7582 | 1071.5019 | 11 | 21.5638 | |
DAFLGSFLYEYSR | Removed: false | 0 | 1566.7355 | | DAFLGSFLYEYSR | Removed: false | light | 2+ | 784.3750 | 1566.7355 | 11 | 29.9827 | |
RHPEYAVSVLLR | Removed: false | 1 | 1438.8045 | | RHPEYAVSVLLR | Removed: false | light | 2+ | 720.4095 | 1438.8045 | 11 | 27.8079 | |
RHPEYAVSVLLR | Removed: false | 1 | 1438.8045 | | RHPEYAVSVLLR | Removed: false | light | 3+ | 480.6088 | 1438.8045 | 11 | 24.4608 | |
HPEYAVSVLLR | Removed: false | 0 | 1282.7034 | | HPEYAVSVLLR | Removed: false | light | 2+ | 642.3590 | 1282.7034 | 9 | 25.1542 | |
HPEYAVSVLLR | Removed: false | 0 | 1282.7034 | | HPEYAVSVLLR | Note: Removed because of lack of precursor signal.
Removed: true | light | 3+ | 428.5751 | 1282.7034 | 9 | 22.1713 | |
EYEATLEECCAK | Removed: false | 0 | 1501.6065 | | EYEATLEECCAK | Removed: false | light | 2+ | 751.8105 | 1501.6065 | 11 | 28.8756 | |
DDPHACYSTVFDK | Removed: false | 0 | 1553.6457 | | DDPHACYSTVFDK | Removed: false | light | 2+ | 777.8301 | 1553.6457 | 11 | 29.7602 | |
DDPHACYSTVFDK | Removed: false | 0 | 1553.6457 | | DDPHACYSTVFDK | Note: Note sure why this was removed.
Removed: true | light | 3+ | 518.8892 | 1553.6457 | 11 | 26.1451 | |
HLVDEPQNLIK | Removed: false | 0 | 1304.7089 | | HLVDEPQNLIK | Removed: false | light | 2+ | 653.3617 | 1304.7089 | 11 | 25.5283 | |
HLVDEPQNLIK | Removed: false | 0 | 1304.7089 | | HLVDEPQNLIK | Note: Removed because fragment signal is higher than precursor signal, but this could be due to lack of charge state separation of the fragments. Might even be an interesting case for IMS.
Removed: true | light | 3+ | 435.9102 | 1304.7089 | 11 | 22.4940 | |
QNCDQFEK | Note: The best precursor peak with idotp=0.99 has noisy product ion signal with only dotp=0.77.
Peptide Quality: Potentially
Removed: true | 0 | 1067.4342 | | QNCDQFEK | Removed: false | light | 2+ | 534.7244 | 1067.4342 | 8 | 21.4946 | |
LGEYGFQNALIVR | Removed: false | 0 | 1478.7882 | | LGEYGFQNALIVR | Removed: false | light | 2+ | 740.4014 | 1478.7882 | 11 | 28.4876 | |
KVPQVSTPTLVEVSR | Removed: false | 1 | 1638.9305 | | KVPQVSTPTLVEVSR | Removed: false | light | 3+ | 547.3174 | 1638.9305 | 11 | 27.3960 | |
VPQVSTPTLVEVSR | Removed: false | 0 | 1510.8355 | | VPQVSTPTLVEVSR | Removed: false | light | 2+ | 756.4250 | 1510.8355 | 10 | 29.0325 | |
CCTKPESER | Note: The most believable precursor ion with idotp=0.99 and mass error 3.6 ppm has poorly formed product ion signal, though dotp=0.87 and mass error 1.3 ppm.
Peptide Quality: Potentially
Removed: true | 0 | 1165.4856 | | CCTKPESER | Removed: false | light | 2+ | 583.7501 | 1165.4856 | 7 | 23.1615 | |
MPCTEDYLSLILNR | Removed: false | 0 | 1723.8273 | | MPCTEDYLSLILNR | Removed: false | light | 3+ | 575.6164 | 1723.8273 | 11 | 28.6411 | |
LCVLHEK | Removed: false | 0 | 897.4742 | | LCVLHEK | Removed: false | light | 2+ | 449.7444 | 897.4742 | 8 | 18.6053 | |
CCTESLVNR | Removed: false | 0 | 1137.4907 | | CCTESLVNR | Removed: false | light | 2+ | 569.7526 | 1137.4907 | 9 | 22.6856 | |
RPCFSALTPDETYVPK | Removed: false | 0 | 1879.9138 | | RPCFSALTPDETYVPK | Removed: false | light | 3+ | 627.6452 | 1879.9138 | 11 | 30.9304 | |
LFTFHADICTLPDTEK | Removed: false | 0 | 1906.9135 | | LFTFHADICTLPDTEK | Removed: false | light | 3+ | 636.6451 | 1906.9135 | 11 | 31.3264 | |
KQTALVELLK | Removed: false | 1 | 1141.7071 | | KQTALVELLK | Removed: false | light | 2+ | 571.8608 | 1141.7071 | 11 | 22.7573 | |
QTALVELLK | Removed: false | 0 | 1013.6121 | | QTALVELLK | Removed: false | light | 2+ | 507.8133 | 1013.6121 | 9 | 20.5797 | |
ATEEQLK | Note: No believable precursor-product combination.
Peptide Quality: Bad
Removed: true | 0 | 817.4181 | | ATEEQLK | Removed: false | light | 2+ | 409.7163 | 817.4181 | 6 | 17.2444 | |
TVMENFVAFVDK | Removed: false | 0 | 1398.6853 | | TVMENFVAFVDK | Removed: false | light | 2+ | 700.3499 | 1398.6853 | 11 | 27.1259 | |
TVMENFVAFVDK | Removed: false | 0 | 1398.6853 | | TVMENFVAFVDK | Note: Removed because of lack of precursor signal.
Removed: true | light | 3+ | 467.2357 | 1398.6853 | 7 | 23.8724 | |
CCAADDKEACFAVEGPK | Removed: false | 1 | 1926.7910 | | CCAADDKEACFAVEGPK | Removed: false | light | 3+ | 643.2709 | 1926.7910 | 11 | 31.6179 | |
EACFAVEGPK | Removed: false | 0 | 1106.5066 | | EACFAVEGPK | Removed: false | light | 2+ | 554.2606 | 1106.5066 | 10 | 22.1589 | |
LVVSTQTALA | Note: Not easy to understand why this was removed, except that its fragments are all b-ions starting with b3. Signal looks acceptable on both precursor with idotp=0.99 a little high mass error of 13.4 ppm, and product signal at 50,000 looks acceptably high and well formed, with dotp=0.82 and mass error of 5.7 ppm.
Peptide Quality: Good
Removed: true | 0 | 1001.5757 | | LVVSTQTALA | Removed: false | light | 2+ | 501.7951 | 1001.5757 | 6 | 20.3750 | |